Medical Clinical Real-time Quantitative PCR Analyzer
YSPCR-Q96 real-time quantitative PCR system is based on global vision of product design concepts and manufacturing processes. It creatively combines Fresnel lens optical signal acquisition technology, time-resolved signal separation technology and unique temperature control technology. And it reaches inteDNAtional advanced level in sensitivity, multi-color crosstalk, temperature uniformity and accuracy. It supports the application of all common QPCR detection modes.
Up to 6 fluorescence detection channels meet the requirement of multiplex PCR
Effectively reduce multi-color crosstalk and edge effect
New optical scanning detection system--High sensitivity /resolution
Innovative scanning method and time-resolved signal separation technology--High accuracy
Unique edge temperature compensation technology
Unique design, user-friendly operating software
One-stop process and solution
Classic examples
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Program:
| Step |
Temperature |
Time |
Cycle |
End point |
| 1 |
42ºC |
20min |
1 |
No |
| 2 |
95ºC |
10min |
1 |
No |
| 3 |
94ºC |
15sec |
40 |
No |
| 55ºC |
30sec |
Yes |
Judgment basis:
Positive: Ct≤35,and the amplification curve has a significant exponential growth curve.
Negative:Ct>37,or no significant amplification of the curve.
Recommended retest: 35
Amplification curve:
Technical Parameters
| Temperature control system |
| Sample capacity |
0.2ml PCR tubes×96, 8×12 PCR plate or 96 well plate ×1 |
| Reaction volume |
10-50 μl |
| Thermal cycle technology |
Peltier |
| Max. Heating/Cooling rate |
6.0° C/s |
| Heating temperature range |
4 - 100 °C |
| Temperature accuracy |
± 0.2°C |
| Temperature uniformity |
±0.2ºC @60ºC , ±0.3ºC @95ºC |
| Temperature gradient setting range |
30-100°C |
| Temperature gradient difference setting range |
1 - 36 °C |
| Detection system |
| Excitation light source |
4/6 monochrome high efficiency LEDs |
| Detection device |
PMT |
| Detection mode |
Time-resolved signal separating technology |
| Excitation/detection wavelength range |
455-650nm/510-715nm |
| Fluorescent channels |
4/6 channels |
| Supported dye |
FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Sensitivity |
Single copy gene |
| Resolution |
1.33 folds copy number difference can be distinguished in single-plex qPCR |
| Dynamic range |
10 orders of magnitude copies |
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