Medical YSPCR-Q96 Real-time Quantitative PCR
YSPCR-Q96 real-time quantitative PCR system is based on global vision of product design concepts and manufacturing processes. It creatively combines Fresnel lens optical signal acquisition technology, time-resolved signal separation technology and unique temperature control technology. And it reaches inte DNAtional advanced level in sensitivity, multi-color crosstalk, temperature uniformity and accuracy. It supports the application of all common QPCR detection modes.
Up to 6 fluorescence detection channels meet the requirement of multiplex PCR
Effectively reduce multi-color crosstalk and edge effect
New optical scanning detection system--High sensitivity /resolution
Innovative scanning method and time-resolved signal separation technology--High accuracy
Unique edge temperature compensation technology
Unique design, user-friendly operating software
One-stop process and solution
Classic examples
Program:
| Step |
Temperature |
Time |
Cycle |
End point |
| 1 |
42ºC |
20min |
1 |
No |
| 2 |
95ºC |
10min |
1 |
No |
| 3 |
94ºC |
15sec |
40 |
No |
| 55ºC |
30sec |
Yes |
Judgment basis:
Positive: Ct≤35,and the amplification curve has a significant exponential growth curve.
Negative:Ct>37,or no significant amplification of the curve.
Recommended retest: 35
Amplification curve:
Technical Parameters
| Temperature control system |
| Sample capacity |
0.2ml PCR tubes×96, 8×12 PCR plate or 96 well plate ×1 |
| Reaction volume |
10-50 μl |
| Thermal cycle technology |
Peltier |
| Max. Heating/Cooling rate |
6.0° C/s |
| Heating temperature range |
4 - 100 °C |
| Temperature accuracy |
± 0.2°C |
| Temperature uniformity |
±0.2ºC @60ºC , ±0.3ºC @95ºC |
| Temperature gradient setting range |
30-100°C |
| Temperature gradient difference setting range |
1 - 36 °C |
| Detection system |
| Excitation light source |
4/6 monochrome high efficiency LEDs |
| Detection device |
PMT |
| Detection mode |
Time-resolved signal separating technology |
| Excitation/detection wavelength range |
455-650nm/510-715nm |
| Fluorescent channels |
4/6 channels |
| Supported dye |
FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Sensitivity |
Single copy gene |
| Resolution |
1.33 folds copy number difference can be distinguished in single-plex qPCR |
| Dynamic range |
10 orders of magnitude copies |
Related products:
Portable PCR gradient thermocycler thermal cycler
Features:
• Widely used in molecular biology, clinical diagnosis, criminal investigations and infectious disease research etc.
• Provides consistent and uniform sealing across an entire block
• Excellent temperature control system
• Fast heating and cooling rate
• Intuitive touch-screen user interface
Specifications
| Sample Capacity |
0.2ml PCR tubes×96, 1PCR plate 8×12 or 96 well plate x1 |
| Heating Temperature Range[oC] |
4-99 |
| Lid Temperature Range[oC] |
50-110 |
| Temperature Display Aaccuracy[oC] |
0.1 |
| Temperature Accuracy[oC@55oC] |
±0.3 |
| Temperature Uniformity[oC@55oC] |
< 0.3 |
| Max. Heating/Cooling Rate [°C /sec] |
3 |
| Display |
7"800×480 LED |
| Touch Operation |
Yes |
| power-off Protection |
Yes |
| Power Supply |
100-120V/200-240V, 50/60Hz |
| |
|
Other model: YSPCR-10S
Thermo Cycler
Thermal cyclers are essential laboratory equipment in the field of biotechnology cloning, genotyping, mutagenesis, and many other applications. The YSPCR-10G has the precise temperature control system and thermal gradient to easily optimize PCR assays in a single run, makes it possible to quickly and accurately obtain large quantities of DNA needed in molecular biology, clinical diagnosis, criminal investigations and infectious disease research etc.
Features:
• Provides consistent and uniform sealing across an entire block
• Excellent temperature control system
• Fast heating and cooling rate
• Large color touch screen for easy programming
• Quickly and easily identify optimal annealing temperatures by using the programmable temperature gradient
Specifications
| Sample Capacity |
0.2mL PCR tubes×96,0.2mL×12 PCR 8 strips or 96 well PCR plate |
| Heating Temperature Range[oC] |
4-99 |
| Lid Temperature Range[oC] |
50-110 |
| Temperature Display Aaccuracy[oC] |
0.1 |
| Temperature Accuracy[oC@55oC] |
±0.3 |
| Temperature Uniformity[oC@55oC] |
< 0.3 |
| Max. Heating/Cooling Rate [°C /sec] |
3 |
| Gradient Temperature |
Yes |
| Gradient Temperature Range[oC] |
30-99 |
| Gradient Range[oC] |
1-42 |
| Display |
7"800×480 LCD |
| Touch Operation |
Yes |
| power-off Protection |
Yes |
| Power Supply |
100-120V/200-240V, 50/60Hz |
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